User manual LEICA APPLICATION SUITE V3.6.0

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Date: April 2010 Issue: 19 Printed in UK Table of Contents 1 Supported Microscopes, Cameras and Accessories 1. 1 1. 2 1. 3 2. 1 2. 2 2. 3 2. 4 2. 5 2. 6 3. 1 3. 2 3. 3 3. 4 3. 5 3. 6 3. 7 3. 8 3. 9 1 DM Microscopes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 Stereo- and Macroscopes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 Cameras. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 2 Computer System (PC) Specification 4 Software Products using this Specification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [. . . ] 8 OASIS-4i Upgrade Kit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 4 Factors that may reduce performance 10 1 Supported Microscopes, Cameras and Accessories The following Leica microscopes and digital cameras are supported. For other Leica Industry division software products please refer to the software product specifications for supported hardware and not this section. For LAS Power Mosaic, please see Section 3 for supported equipment. 1. 1 DM Microscopes DM1000, 2000, 2500, 3000, 4000 B/M, 4500 B, 4500 P, 5000 B, 5500 B, 6000 B/M DMI4000 B, 5000 M and 6000 B DM500, DM750, DM750 P FSC, FSM CTR 4000-6500 Accessories for DM Scanning stages External light source EL6000 External filter wheels TIRF module Motorized duo documentation tube Oasis-blue XYZ controller 1. 2 Stereo- and Macroscopes DVM2000 DVM3000 and DVM5000 (for firmware update only) LED illumination LED3000 RL (ring light) KL1500 LED Plus LED illuminations LED5000 CXI (coax) and LED3000 NVI (near vertical) Manual stereomicroscopes M50, M60 and M80 M205A, fully motorized stereomicroscope with 20. 5:1 zoom range M205FA, fully motorized fluorescence stereomicroscope with 20. 5:1 zoom range M165FC, manual fluorescence stereomicroscope with coded filter wheel, zoom and iris and 16. 5:1 zoom range M125, manual stereomicroscope with 12. 5:1 zoom range M-Series Long / Short Motor Focus Columns (10450258 and 10450050) M-Series Long / Short Focus Columns (10450257 and 10450022) FluoCombiTM attachment for fluorescence stereomicroscope with coded objective positions Fluorescence objective revolver with coded objective positions Coded Stereo Zooms M165C and M205C LED illumination for VZ series (10450433 and 10450432) LED illumination LED5000 MCI, LED5000 HDI and LED5000 RL-80/48 LAS System Requirements Page 1 USB interface for new manual focus drive coarse/fine MZ-Series Long / Short Motor Focus Columns (10446041 and 10446176) MZ 16A and MZ 16FA Z 6 APOA and Z 16 APOA IsoProTM motorized XY stage Configuration of new footswitch Configuration of UMC MZ 16F, MZ 16 MZ 12. 5 MZ10 F MZ 9. 5, MZ 7. 5, MZ 6 MS 5 S6D S8 APO Z6 APO, Z16 APO VZ85, VZ85 R, VZ85 C, VZ85 RC, VZ105, VZ105 C, VZ15 M EZ4 D (NOT Windows Vista, Windows 7) MacrofluoTM Fluocombi / Revolving nosepiece Support by USB for TL RCI and IsoProTM motorized XY stage Support for USB / RS 232 serial adapter (Art. 11532295) Transmitted light base for Stereo Microscopes TL RCITM External light sources (Photonic CLS 150XD, CLS 150LS, Schott KL 2005 LCD, EL6000) Oasis-blue XY controller (not Power Mosaic) LAS System Requirements Page 2 1. 3 Cameras Support for Windows XP, Windows Vista and Windows 7 This release supports the following Leica Digital Cameras on a Windows PC meeting the requirements detailed in Section 2: FireWire A (1394a) DFC280 DFC290 DFC320 DFC300 FX DFC340 FX DFC350 FX DFC420 DFC420 C DFC480 DFC490 IC D IC 3D DFC500 FireWire B (1394b) DFC295 DFC290 HD DFC310 FX DFC345 FX DFC360 FX DFC400 DFC425 DFC425 C DFC495 DVM2000 USB2 IC80 HD ICC50 NOT Supported LAS does not support the following: Matrox Meteor 2 or Leutron frame grabbers Leica EC3 Leica DFC300, DFC300F, DFC350F Leica DC100, DC150, DC160, DC180, DC200, DC250, DC300, DC300F, DC300FX, DC350F, DC350FX, DC480, DC500 LAS System Requirements Page 3 2 Computer System (PC) Specification 2. 1 Software Products using this Specification PC Specification for LAS software This PC specification is targeted at Leica LAS software V3 or higher. Lower specifications may limit the performance and some features of LAS. If using Windows Vista or Windows 7, a Windows experience index of 3. 5 is required for simple acquisition and 4. 5 or higher where multiple images are being processed. Other Leica Software using this PC Specification This PC specification also applies to the following software products with the restrictions mentioned: Leica Servers - only with Windows XP and Vista 32-bit Leica QWin - only with Windows XP and Vista 32-bit Leica Materials Workstation - only with Windows XP and Vista 32-bit Leica Stereo Explorer ­ only with Windows XP 32-bit Leica IM ­ only with Windows XP 32-bit For all other software products, please refer to their individual specifications. Leica LAS EZ software LAS EZ does not require a Firewire interface. Minimum RAM of 1Gb combined with the other specification details as follows is acceptable. Not for use with Windows 7. Leica DFC Twain software The PC specification will depend on the requirements of the host software application and the suppliers own specifications for their software should be followed. The specification following is typical. 2. 2 Processor Intel Core 2 Duo with nominal CPU clock > 2GHz or Intel Pentium 4 32-bit (x86) Processor with nominal CPU clock of > 3. 0GHz and including at least: 2MbL2 cache 800 Mhz FSB 2GB or higher of system RAM is strongly recommended. Where application is limited to LAS Core and Windows XP, 1GB may be acceptable. Hard Drive: 80GB or higher, SATA 7200 rpm, 8MB cache, NTFS Display and Graphics - 19 or greater size monitor, one or two monitors, set to screen resolution of 1920 x 1200 or 1600 x 1200 or 1280 1024 Graphics card minimum parameters: 128Mb Ram minimum for single screen or 256Mb Ram for dual screen 128-bit memory bus width 32 bits operation Support for Direct X Version 9c or Direct X Version 10 for Windows Vista or Windows 7. PCI Express LAS System Requirements Page 4 The recommended minimum graphics adaptors are the Radeon X800, ATI Fire GL V7200or a recent NVidia Quadro graphics adaptor using the PCI Express bus or later models. `Integrated' graphics cards may have reduced performance and are not recommended. Use of system or shared memory by the graphic card will impair performance. It is vital that the manufacturer's latest graphics drivers are installed for LAS to display images and the user interface correctly and particularly for use with the LAS 3D Viewer and LAS MultiTime ­ Movie modules. Obtain these from the PC or graphics board manufacturer, not Microsoft Corp. 2. 3 Interfaces Leica DFC Cameras Depending on the type of camera, a powered 1394a 6-pin or 1394b 9-pin FireWire OHCI port is required for use with Leica DFC cameras. As FireWire interface boards from some manufacturers have issues with non-Leica drivers, we recommend the use of the FireWire boards in the list below. Please note that you will also need the FireWire-b-b cable 12730186, if both camera and PC have a 1394b or FireWire-b connector. The following cards are recommended: Manufacturer IOI Zenkuman IOI Model FWB-PCI 02 PFW-85 FWB-PCIE 1X21B 107355 Ports 1394b 2-port 1394b PCI-64 2-port 1394b PCI-32 3-port 1394b PCI Express 1394a 3+1 port 1394a PCI-32 Chipset Texas Instruments Texas Instruments Agere Leica Article Nr 11 600 274 36 000 191 12 730 210 LaCie VIA 12730183 All DFC cameras are supplied with a suitable FireWire cable. [. . . ] For the upright stands, the following are recommended: 12 730 196 Märzhäuser Scanning Stage, 100x80mm, 2mm with Encoders Requires 12 730 195 Oasis-blue Encoder kit for Power Mosaic 12 730 197 Märzhäuser Scanning Stage, 100x80mm, 2mm At least one stage insert is also required: 12 730 198 Stage insert for one slide for SCAN 130 x 85 12 730 199 Stage insert for four slides for SCAN 130 x 85 The Smart Move controls the stage and focus - the focus knob on the microscope stand is disabled. 11 505 180 Smart Move for DM/DMI Series 10 450 218 IsoPro 6x4" motorized xy-stage V2 ­ note this stage is the new V2 stage that includes encoders. 3. 7 Motorized Focus DM5500-6000 use internal focus motor Driven by Oasis using CTR MIC control cable with Z breakout. DM4000 use external Märzhäuser focus drive Included with 11 101 938 DM4000 M stand 3. 8 Encoder Option 12 730 195 Oasis-blue Encoder kit for Power Mosaic Used where best accuracy is required with high-mag objectives Daughter board that fits on the Oasis-Blue board Modified XY Cable for Encoder Inputs Requires 12 730 196 Märzhäuser Scanning Stage, 100x80mm, 2mm with Encoders 3. 9 OASIS-4i Upgrade Kit Enables customers with previous generation Oasis boards to upgrade Assumes that the microscopes and stage control is already functioning LAS System Requirements Page 8 12 730 194 Kit includes OASIS-DC1 daughter board DFC Camera Trigger Cable Does not include Smart Move kit. Note ­ Oasis-4i will require a license code to be supplied, please contact DI. Support for this. LAS System Requirements Page 9 4 Factors that may reduce performance The following is a non-exclusive list of PC components that may degrade the performance of LAS. [. . . ]