User manual FLUORESCENCE VC-CATALOG

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[. . . ] Visit our website for decriptions of other series. Inlet and outlet tubes can be either 1/16 (standard) or 1/8 in. Tubes are available in steel or plastic, and in a variety of ID's. ID tubing are available as an option. Several flow patterns are available. The flow through this laminar perfusion chamber is shown by arrows. Body diameter is 1. 12 inches. [. . . ] 1 Coverslips, 1 Box VC-48x65CS-1 VC-RPC-LW-ORINGS Replacement O-rings for RPC-LW, 5 Sets C&L Instruments, Inc. Patent Pending www. fluorescence. com 717-564-9491 Vacu-CellTM System Rectangular Perfusion Chamber, Thin Window Model RPC-TW This Perfusion Chamber uses two rectangular coverslips to form a thin perfusion chamber. Two sets of dual o-rings on the bottom of the chamber body securely hold a 24 x 40 mm (upper) and a 48 x 65 mm (lower) coverslip in place. Each coverslip is held in place using a separate vacuum port, so they can be used independently. When the Model RPC-TW is used with two coverslips, a rectangular laminar flow chamber is formed with the two coverslips separated 100 microns, creating a thin laminar flow chamber. Inlet and outlet perfusion ports direct fluid flow from one end to the other between the two coverslips in a laminar fashion. Either the upper or lower coverslip can be used without the other to fashion an open chamber configuration. This chamber is available in black anodized aluminum that is coated with parylene for biological compatibility. Top and bottom vacuum ports The observation window measures 0. 44 x 1. 04 inches (bottom view, without coverslips attached) Model VC-RPC-TW Model VC-RPC-TW (top view) Model Numbers VC-RPC-TW-BA VC-48x65CS-1 VC-24x40CS-1 VC-RPC-TW-ORINGS Thin Window RPC, Black Anodized Aluminum Rectangular 48 x 65 mm No. 1Coverslips, 1 Box O-rings for RPC-TW, 5 Sets Model VC-RPC-TW (sectioned view) C&L Instruments, Inc. www. fluorescence. com 717-564-9491 Patent Pending Vacu-CellTM System Mini Perfusion Chamber, Open Top Model MPC-OT The Mini Series Open Top Chamber uses a single 25 mm round coverslip. Dual o-rings on the bottom of the chamber securely hold the coverslip in place using a vacuum seal. The total volume in the observation window is approximately 240 µl when the depth of the perfusion fluid is 1 mm. This chamber is available in black anodized aluminum that is coated with parylene for biological compatibility. Temperature is controlled using a heated microscope stage adapter. Vacuum port The observation window measures 17. 5 mm diameter Model VC-MPC-OT (sectioned view) Perfusion port Model VC-MPC-OT (top view) Model Numbers VC-MPC-OT-BA VC-25RCS-1 VC-25RCS-2 VC-MPC-OT-ORINGS Large Window MPC, Black Anodized Aluminum 25 mm No. 2 Round Coverslips, Box of 110 O-rings for MPC-OT, 5 Sets C&L Instruments, Inc. Patent Pending www. fluorescence. com 717-564-9491 Vacu-CellTM System Mini Perfusion Chamber, Large Window Model MPC-LW The Mini series Large Window Perfusion Chamber uses two round 25 mm coverslips to form perfusion chamber. Dual o-rings on the bottom and top of the chamber body securely hold the coverslips in place. Each coverslip is held using a separate vacuum port, so they can be used independently. When the Model MPC-LW is used with two coverslips, a circular chamber is formed with the two coverslips spaced by 3. 7 mm. The total volume in the observation window is approximately 890 µl. The perfusion ports direct fluid flow from one end to the other between the two coverslips. The lower coverslip can be used without the upper coverslip to fashion an open chamber configuration, allowing access to the coverslip surface. The top coverslip can be added at any time to close the chamber for perfusion. This perfusion chamber works well using either an inverted or standard microscope. [. . . ] A vacuum gauge measures the vacuum available on the output port. Model Numbers VCS-1 Vacuum Pump System 110-120 or 220-240 VAC (specify on order) C&L Instruments, Inc. www. fluorescence. com 717-564-9491 Vacu-CellTM System Temperature Controller Model TC-30 The Model TC-30 temperature controller is designed to precisely control the temperature of a Vacu-Cell incubation chamber or a Vacu-Cell microscope stage holder. The heater provides a heavily filtered DC output at a maximum power of 30 Watts for noise-free temperature control. A highly visible digital temperature readout indicates either the set-point temperature of the controller or the measured temperature of the sensor probe. The gain control is used to adjust the feedback system and the dynamic temperature response of the Vacu-Cell. [. . . ]

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